The government is spending $3.1 billion buying up water rights across the Murray-Darling Basin.
More than 350 irrigators in the southern Murray-Darling Basin have offered up their water rights to the federal government.
The $50 million tender closed on Wednesday.
The government is spending $3.1 billion buying up farmers' water rights across the basin, in a bid to boost the environment in dry times.
Rob Freeman, chief executive of the Murray Darling Basin Authority, said the latest tender round had received a "high level of interest" and was now fully subscribed.